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Banana dna extraction ( or “ how to make the best banana smoothie ever” ) dna is a nucleic acid located in the cell' s nucleus. in the plastic cup, mix a solution of 1 pdf tsp. mix in the blender for 5 to 10 seconds making sure the mixture is not too runny. you can also use a coffee filter, or a funnel with a layer or two of toilet or kitchen tissue.
place your banana pieces in the blender, add a teaspoon of salt and slightly cover the mixture with warm water. keep the alcohol and liquefied banana as separate as possible. with a fine mesh, extract the liquid into cup # 3. dna can be extracted and observed. lane 1- 7: banana dna extraction results and discussion pdf genomic dna isolated from different banana cultivars. the experiment is then carried out to extract the dna from a banana. it uses strawberries instead of bananas. the gaps in between stages can be discussion used to further discuss genetics and inheritability. discussion & conclusion banana plants are economically important discussion due to its nutritional value.
transfer to a tall transparent container the ideal container would be a big test tube, but if results you discussion don’ t have one, try to get the thinnest glass jar you can find. with a few simple household items, you can isolate the dna of bananas in your kitchen. lab practice: extracting dna from a banana teacher’ s summary: this short activity helps students visualize one of the most important molecules on the planet, dna. results & discussion 4. blend or mash the banana and salt with the water to make a slurry b.
first you will need to put the 1⁄ 2 banana and 1⁄ 4 c distilled water in the plastic bag, seal the bag and mash them to make your slurry. let the bag sit for 5 minutes. using your knife, cut your banana into tiny pieces to expose more of the cells. one in particular we like is by nih – genome unlocking life’ s code. org/ node/ 653 overview. the experimental compo- nent has multiple stages, some of which do in- volve waiting before the next stage.
there are many dna extraction protocols that can be found on the web. materials • 3 cm piece of a ripe banana. add 2ml dishwashing detergent to each flask of banana/ salt solution a. we use bananas in the text, results but strawberries or other fruit soft enough to mush up can also be used instead. the activity can be done with simple materials found in most homes. results: the optimal sodium chloride salt concentration for dna extraction from banana fruits was 4mm and the amount of dna extracted pdf increased as the temperature decreased. banana dna discussion extraction lab objectives: describe where dna is located in a plant cell.
banana dna experiment watch on. let this two- layered mixture sit for eight minutes. this short overview covers various physical and chemical. dna extraction makes it possible to study the genetic causes discussion of diseases and for the development of diagnostics and drugs. the nuclear and the cell membranes are a tough protective barrier, made of lipids and proteins, which need to be banana dna extraction results and discussion pdf eliminated in order to. summarize the main steps involved in extracting dna from bananas. distribute in erlenmeyer flasks lysis ( detergent) 2. even though we might not. of a banana 1 drop of dish soap tiny pinch of salt 3- 4 tbsp water. describe the appearance of the dna you extracted.
swirl gently to mix without creating a lot of bubbles. therefore, it is crucial to preserve genetic material of musa species for their sustainable exploitation. in this activity, students extract dna ( and also some rna) from bananas. it is found making up the genetic material and pdf is bound to several types of proteins. summary: the lesson/ workshop plan has two parts:.
the sciences key concepts cells dna genes from national science education standards: reproduction and banana dna extraction results and discussion pdf heredity introduction what do you have in common with a banana? name: _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ my mark: _ _ _ _ _ _ _ _ _ _ _ / 15 teacher mark: _ _ _ _ _ _ _ / 15 full of dna. spin it in place so that cloudy layer spools around it. at present several banana varieties have many. omara and barugahare; jabb, 25( 4) : 1- 5, ; article no. pdf the longer you wait, the more defined this layer becomes.
the first method requires an online # banana dna extraction# protocol to extract the banana# results s dna from its content. dna extraction and polymerase chain reaction ( pcr) are the basic techniques employed in the molecular laboratory. do you think your. stick the wooden stirrer into the cup. questions write your answers on a separate sheet of paper. they see that: dna is a component of living and once- living things. as in bananas, dna is results located within the cell in an even smaller com- partment, the cell nucleus.
put the above in a ziploc bag and mash it together. 5 6 other options this is our banana dna extraction results and discussion pdf spin on a classic activity. as for the pdf second procedure, it involves analyzing the dna with a centrifuge, vortex, and. 1/ 2 tsp liquid dishwashing soap resealable zip- top bag ( quart size) very cold results rubbing alcohol ( isopropyl pdf alcohol) placed in freezer ahead of time coffee filter narrow glass wooden banana dna extraction results and discussion pdf stirrer watch biologist melissa wilson sayres as she shows you step- by- step how to extract dna from a banana. introduction dna extraction is the process by which the dna is purified from the living or dead cells or tissues. this is the dna pieces clumping together. in doing so, you follow the same principles as the biological researchers when they isolate dna in the laboratory. although concentrations were low, clear bands were still obtained from the amplifications. sequencing genome, detecting bacteria, and viruses in the environment and for detecting paternity become possible with the help of dna extraction. shampoo, 2 pinches of salt and 4 tsp.
strain through sieve to remove large chunks c. the salt will help the dna stay together during the mashing process. dna extraction also becomes an essential process in field of forensic science. stir this solution slowly for about a minute until the shampoo dissolves in the water. this method requires primarily of an antibacterial hand soap, ethanol, pipettes, mortar, pestle, salt, coffee filters, and beakers. use this time to either discuss what dna might look like or why you added the salt and detergent and are mashing it. explain what procedures are required to release dna from a plant cell observe the extraction of genomic dna from plant cells. 1 banana, ml cold water, 1g table salt a. dna extraction the concentrations of the extracted dna was measured using the nanophotometer™, uv/ vis pdf spectrophotometer ( implen, munich, germany) and the average concentration for 12 samples was 0.
